Micellar Liquid Chromatographic Method for Determination of Moxifloxacin and its Impurities

A selective eco-friendly micellar HPLC method was developed for investigation of moxifloxacin and related compounds in the presence its degradation products. Central composite design used to optimize experimental conditions. The proposed is based on isocratic elution a C18 column using 92.5% (v/v) biodegradable aqueous mobile phase containing 0.01 M sodium dihydrogen phosphate, 0.15 dodecyl sulfate (SDS) 0.5% triethylamine with pH 3.5 7.5% isopropanol as eco- friendly organic solvent. flow rate injection volume were 0.6 ml/min 5 μl, respectively. Experiments performed at temperature 60 °C detection 295 nm. optimized validated. found be suitable quantification drug substance. Green Analytical Procedure Index (GAPI) proves superiority against other reported methods.